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KMID : 0356820170330010021
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Korean Journal of Head & Neck Oncology
2017 Volume.33 No. 1 p.21 ~ p.29
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Genome-wide Methylation Analysis and Validation of Cancer Specific Biomarker of Head and Neck Cancer
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Chang Jae-Won
Park Ki-Wan
Hong So-Hye
Jung Seung-Nam
Liu Lihua
Kim Jin-Man
Oh Tae-Jung
Koo Bon-Seok
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Abstract
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Methylation of CpG islands in the promoter region of genes acts as a significant mechanism of epigenetic gene silencing in head and neck squamous cell carcinoma (HNSCC). DNA methylation markers are particularly advantageous because DNA methylation is an early event in tumorigenesis, and the epigenetic modification, 5-methylcytosine, is a stable mark. In the present study, we assessed the genome-wide preliminary screening and were to identify novel methylation biomarker candidate in HNSCC. Genome-wide methylation analysis was performed on 10 HNSCC tumors using the Methylated DNA Isolation Assay (MeDIA) CpG island microarray. Validation was done using immunohistochemistry using tissue microarray of 135 independent HNSCC tumors. In addition, in vitro proliferation, migration/invasion assays, RT-PCR and immunoblotting were performed to elucidate molecular regulating mechanisms. Our preliminary validation using CpG microarray data set, immunohisto-chemistry for HNSCC tumor tissues and in vitro functional assays revealed that methylation of the Homeobox B5 (HOXB5) and H6 Family Homeobox 2 (HMX2) could be possible novel methylation biomarkers in HNSCC.
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KEYWORD
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Genome-wide methylation analysis , Methylation biomarker , Homeobox B5 (HOXB5) , H6 Family Homeobox 2 (HMX2) , Head and neck squamous cell carcinoma (HNSCC)
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